题目：Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome 

报告人：闫凯歌博士 MRC Laboratory of Molecular Biology，Investigator Scientist

时间： 2020年1月8日 14:30

地点：生命科学楼531会议室 

Abstract:Kinetochores are large protein complexes that attach chromosomes, through their centromeres, to the mitotic spindle. By assembling onto the specialized centromeric Cenp-A nucleosome, kinetochores provide load-bearing structures for microtubule-mediated chromosome segregation in mitosis. The inner and outer kinetochores are responsible for interacting with centromeric chromatin and microtubules, respectively. We have reconstituted the inner kinetochore onto a specialised Cenp-A nucleosome. I will describe the cryo-EM structure of the S. cerevisiae inner kinetochore 14-subunit CCAN complex assembled onto a Cenp-A nucleosome. The structure explains the inter-dependency of CCAN constituent sub-complexes and how the inner kinetochore interacts with the octameric Cenp-A nucleosome. The CCAN–Cenp-A structure provides a framework for understanding how kinetochores link centromeres to microtubules for chromosome segregation.