研究方向与兴趣：
生殖细胞发育异常引起的人类生殖障碍及出生缺陷是我国面临的严重人口健康挑战。当前，由于生活/饮食方式的改变，环境污染的存在，据统计我国育龄夫妇中约有10~15%不能正常生育，且在每年出生的新生儿中，约有3~6%存在先天出生缺陷。这些给我国人口健康带来巨大挑战和社会负担。本研究组将通过制作基因修饰的小鼠动物模型，综合利用细胞生物学与分子生物学技术，并结合人类临床病理标本，从遗传和表观遗传角度来探讨哺乳动物生殖细胞发育、分化、受精及环境获得性性状子代传递(Acquired traits)的分子机制。
特任研究员 博士生导师2010年获得上海交通大学医学院生殖生物学博士学位，2011~2018年分别在美国University of Nevada Reno和University of Texas MD Anderson Cancer Center接受博士后训练。目前实验室主要围绕1）建立低起始量样本(low-input)的灵敏、高通量组学技术，来探索、解决生殖生物学前沿科学问题；2）开发优化高效、精准的长片断基因敲入（Knock-in）技术方法，用于细胞、小鼠动物模型的制作，以及先天遗传疾病/出生缺陷的诊治；
近5 年作为通讯作者相关成果发表在Nature Communications, Nucleic Acids Research, PNAS, eLife，WIREs RNA等。目前担任安徽省细胞生物学会生殖医学分会主任委员等学术兼职！
论文发表链接：
Google Scholar: https://scholar.google.com/citations?user=M582R4cAAAAJ&hl=en
近五年发表SCI论文：
1: Azhar M, Xu C, Jiang X, Li W, Cao Y, Zhu X, Xing X, Wu L, Zou J, Meng L, Cheng Y, Han W, Bao J. The arginine methyltransferase Prmt1 coordinates the germline arginine methylome essential for spermatogonial homeostasis and male fertility. Nucleic Acids Res. 2023 Sep 22:gkad769. doi: 10.1093/nar/gkad769. Epub ahead of print. PMID: 37739418.
2: Jiang X, Cheng Y, Zhu Y, Xu C, Li Q, Xing X, Li W, Zou J, Meng L, Azhar M, Cao Y, Tong X, Qin W, Zhu X, Bao J. Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice. Nat Commun. 2023 Jun 22;14(1):3729. doi: 10.1038/s41467-023-39256-0. PMID: 37349316; PMCID: PMC10287700.
3: Huang L, Li W, Dai X, Zhao S, Xu B, Wang F, Jin RT, Luo L, Wu L, Jiang X, Cheng Y, Zou J, Xu C, Tong X, Fan HY, Zhao H, Bao J. Biallelic variants in MAD2L1BP (p31comet) cause female infertility characterized by oocyte maturation arrest. Elife. 2023 Jun 19;12:e85649. doi: 10.7554/eLife.85649. PMID: 37334967; PMCID: PMC10319434.
4: Han W, Li Z, Guo Y, He K, Li W, Xu C, Ge L, He M, Yin X, Zhou J, Li C, Yao D, Bao J, Liang H. Efficient precise integration of large DNA sequences with 3'-overhang dsDNA donors using CRISPR/Cas9. Proc Natl Acad Sci U S A. 2023 May 30;120(22):e2221127120. doi: 10.1073/pnas.2221127120. Epub 2023 May 22. PMID: 37216515; PMCID: PMC10235934.
5: Wang Y, Zhang Y, Yu N, Li B, Gong J, Mei Y, Bao J, Ma S. Decoding transcriptional regulation via a human gene expression predictor. J Genet Genomics. 2023 May;50(5):305-317. doi: 10.1016/j.jgg.2023.01.006. Epub 2023 Jan 21. PMID: 36693565.
6: Cao Y, Meng L, Cheng Y, Azhar M, Jiang X, Xing X, Bao J. Optimized protocol for isolation of germ cells from mouse testis by centrifugal elutriation. STAR Protoc. 2022 Sep 16;3(3):101540. doi: 10.1016/j.xpro.2022.101540. Epub 2022 Jul 13. PMID: 35834386; PMCID: PMC9293666.
7: Xu C, Cao Y, Bao J. Building RNA-protein germ granules: insights from the multifaceted functions of DEAD-box helicase Vasa/Ddx4 in germline development. Cell Mol Life Sci. 2021 Dec 18;79(1):4. doi: 10.1007/s00018-021-04069-1. PMID: 34921622.
8: Jiang X, Zhu X, Cheng Y, Azhar M, Xing X, Li W, Cao Y, Shi Q, Bao J. The Spin1 interactor, Spindoc, is dispensable for meiotic division, but essential for haploid spermatid development in mice. Reprod Biol Endocrinol. 2021 Sep 15;19(1):144. doi: 10.1186/s12958-021-00828-8. PMID: 34526015; PMCID: PMC8442334.
9: Azhar M, Altaf S, Uddin I, Cheng J, Wu L, Tong X, Qin W, Bao J. Towards Post- Meiotic Sperm Production: Genetic Insight into Human Infertility from Mouse Models. Int J Biol Sci. 2021 Jun 16;17(10):2487-2503. doi: 10.7150/ijbs.60384. PMID: 34326689; PMCID: PMC8315030.
10: Altaf S, Bao J. Exome sequencing shines in empty follicle syndrome: zona pellucida gene mutations manifest genuine empty follicle syndrome. Fertil Steril. 2021 May;115(5):1170-1171. doi: 10.1016/j.fertnstert.2021.03.007. Epub 2021 Mar 26. PMID: 33775396.
11: Xu Q, Zhu X, Maqsood M, Li W, Tong X, Kong S, Wang F, Liu X, Wei Z, Zhang Z, Zhu F, Cao Y, Bao J. A novel homozygous nonsense ZP1 variant causes human female infertility associated with empty follicle syndrome (EFS). Mol Genet Genomic Med. 2020 Jul;8(7):e1269. doi: 10.1002/mgg3.1269. Epub 2020 Apr 23. PMID: 32329253; PMCID: PMC7336750.
12: Zhang B, Ma H, Khan T, Ma A, Li T, Zhang H, Gao J, Zhou J, Li Y, Yu C, Bao J, Ali A, Murtaza G, Yin H, Gao Q, Jiang X, Zhang F, Liu C, Khan I, Zubair M, Hussain HMJ, Khan R, Yousaf A, Yuan L, Lu Y, Xu X, Wang Y, Tao Q, Hao Q, Fang H, Cheng H, Zhang Y, Shi Q. A DNAH17 missense variant causes flagella destabilization and asthenozoospermia. J Exp Med. 2020 Feb 3;217(2):e20182365. doi: 10.1084/jem.20182365. PMID: 31658987; PMCID: PMC7041708.
13: Avasarala S, Wu PY, Khan SQ, Yanlin S, Van Scoyk M, Bao J, Di Lorenzo A, David O, Bedford MT, Gupta V, Winn RA, Bikkavilli RK. PRMT6 Promotes Lung Tumor Progression via the Alternate Activation of Tumor-Associated Macrophages. Mol Cancer Res. 2020 Jan;18(1):166-178. doi: 10.1158/1541-7786.MCR-19-0204. Epub 2019 Oct 16. PMID: 31619507; PMCID: PMC6942249.
14: Jiang X, Yin S, Fan S, Bao J, Jiao Y, Ali A, Iqbal F, Xu J, Zhang Y, Shi Q. Npat-dependent programmed Sertoli cell proliferation is indispensable for testis cord development and germ cell mitotic arrest. FASEB J. 2019 Aug;33(8):9075-9086. doi: 10.1096/fj.201802289RR. Epub 2019 May 14. PMID: 31084574.
15: Bao J, Di Lorenzo A, Lin K, Lu Y, Zhong Y, Sebastian MM, Muller WJ, Yang Y, Bedford MT. Mouse Models of Overexpression Reveal Distinct Oncogenic Roles for Different Type I Protein Arginine Methyltransferases. Cancer Res. 2019 Jan 1;79(1):21-32. doi: 10.1158/0008-5472.CAN-18-1995. Epub 2018 Oct 23. PMID: 30352814; PMCID: PMC6714580.
招生招聘：
实验室招聘副研究员、助理研究员，常年招收博士后和研究生，享受中国科学技术大学及附一院相关福利待遇。欢迎有志于从事生殖发育生物学，或有丰富分子生物学，细胞生物学，生物信息学技术的优秀同仁加入，同时热忱欢迎对本实验室研究方向感兴趣的本科生前来攻读博士学位。
联系方式：
科大中区医学楼407（办公室）或408（实验室）
Email: BaoLab@ustc.edu.cn
Office: 0551 63606389
Lab: 0551 63607625